Porcine Toxoplasmosis IgG Antibody ELISA test kit
Catalog No. LSY-30001
1. Usage
Porcine Toxoplasmosis IgG antibody test kit is used for the detection of the Toxoplasmosis antibodies in porcine serum.

2. Principle
The test kit is enzyme immunoassay for the detection toxoplasmosis antibodies in porcine serum. Toxoplasmosis antigens are coated on 96-well plate, add sample into wells and then antibodies bound specifically by antigens, the unbound antibodies and other components will be removed by washing. Next add enzyme conjugation to specifically bind with the compound of antibodies and antigens on the microplate. The unbound enzyme conjugation will be removed by washing. Add the TMB substrate in the well, react to a blue product. At last, end the reaction by adding stop solution.

3. Components
1 Antigen coated microplate 2 pieces (96 wells each)
2 TOX-IgG Negative control serum (green lid) 1.5 mL/tube
TOX-IgG Positive control serum (red lid) 1.5 mL/tube
3 Enzyme conjugate (yellow lid) 1 bottle (22 mL/bottle)
4 20×concentrated washing solution (white lid) 1 bottle (50 mL/bottle)
5 Substrate A (purple lid)/B (yellow lid) 1 bottle each (12 mL/bottle)
6 Stop solution (blue lid) 1 bottle (12 mL/bottle)
7 Sample diluent solution (transparent lid) 1 bottle (25 mL/bottle)
8 Adhesive Foil 6 pieces
9 Instruction 1 piece
(Note: Never mix up reagents from test kits in different batch number)

4. Material Required not Provided
1 Microplate Reader (single-wave length: 450nm or double-wave length: 450/630 nm).
2 Microplate Washer.
3 Micropipettes, adjustable.
4 Constant temperature box or water bath box.
5 Oscillator.

5. Sample requirement
1 The samples are porcine serum, which should be collected with no bacteria. The storage time should be less than 1 week at 2-8 ℃, if for long term, it should be kept at -20℃.
2 Avoid to use the samples with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.
3 The EDTA, heparin sodiun and other anticoagulants will not affect the results.